Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [P/T1] to TGF alpha
Suitable for: IHC-P, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-TGF alpha antibody [P/T1]
See all TGF alpha primary antibodies
Description

Mouse monoclonal [P/T1] to TGF alpha
Host species

Mouse
Specificity

This antibody was selected and evaluated immunoserologically for recombinant human TGF-alpha and synthetic (uncoupled) peptides using simple binding and competitive ELISA. Immunohistochemical characterization of the anti-TGF-alpha monoclonal antibody was made on different human carcinomas. Recommended for immunohistochemical detection of TGF-alpha production in human tumor cells, immunoserological monitoring of progression of malignant tumours and for screening of therapeutic efficiency of different human tumors.
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide fragment (32-40 amino acids sequence of 50 amino acids) of transforming growth factor alpha (TGF-alpha).
General notes

Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.097% Sodium azide
Constituents: Tris HCl, 0.2% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

P/T1
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [P/T1] (ab9578)

Immunohistochemical characterization of the anti-TGF-a monoclonal antibody ab9578 on human melanoma.
Flow Cytometry - Anti-TGF alpha antibody [P/T1] (ab9578)

Overlay histogram showing A431 cells stained with ab9578 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9578, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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