Anti-Smad2 + Smad3 antibody (ab217553)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Smad2 + Smad3 antibody
See all Smad2 + Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad2 + Smad3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
-
Immunogen
Synthetic peptide within Human Smad2 + Smad3 aa 30-80 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q15796 -
Positive control
- Mouse lymphoma tissue; mouse brain lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Smad2 + Smad3 antibody (ab217553)All lanes : Anti-Smad2 + Smad3 antibody (ab217553) at 1/200 dilution
Lane 1 : mouse brain lysates
Lane 2 : mouse kidney lysates
Secondary
All lanes : HRP-conjugated secondary antibody at 1/3000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?12% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 + Smad3 antibody (ab217553)Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse lymphoma tissue labeling Smad2 + Smad3 with ab217553 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
