Anti-Smad2 + Smad3 antibody (ab63672)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Smad2 + Smad3 antibody
See all Smad2 + Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad2 + Smad3 -
Host species
Rabbit -
Specificity
ab63672 detects endogenous levels of total Smad2 and Smad3. -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
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Immunogen
Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad2 + Smad3 antibody (ab63672)All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts treated with UV (15mins)
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts untreated
Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size: 48 kDa why is the actual band size different from the predicted?
