Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19867] to TGF beta 1+TGF beta 3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free
    See all TGF beta 1+TGF beta 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR19867] to TGF beta 1+TGF beta 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251436 is the carrier-free version of ab205604. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251436 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 3 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
    Lane 4 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab205604, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-3: 30 seconds; Lane 4: 3 minutes.

  • Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/5000 dilution
    Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab205604, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).

  • Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab205604, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).

  • Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/10000 dilution
    Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : Recombinant protein fragment human TGF beta 1
    Lane 2 : Recombinant protein fragment human TGF beta 2
    Lane 3 : Recombinant protein fragment human TGF beta 3

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab205604, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 3 minutes; Lane 3: 15 seconds.

    Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house.

  • Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)
    Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (ab251436)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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