Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade – BSA and Azide free (ab272458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23309-113] to RUNX1 / AML1 - ChIP Grade – BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade – BSA and Azide free
    See all RUNX1 / AML1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23309-113] to RUNX1 / AML1 - ChIP Grade – BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ChIPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, MOLT-4 and THP-1 whole cell lysates. Flow Cyt: Jurkat cells. IP: Jurkat cells.ChIP: Chromatin prepared from K562 cells.

  • General notes

    ab272458 is the carrier-free version of ab272456. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)
    ChIP - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)

    Chromatin was prepared from K-562 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab272456 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are from paper PMID：20959602

    *https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    The RUNX1 enrichment profile is consistent with what have been described in literature (PMID: 20959602).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272456).

  • Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)
    Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)

    RUNX1 / AML1 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab272456 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab272456 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 20 ug

    Lane 2: ab272456 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272456 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272456).

  • Flow Cytometry - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)
    Flow Cytometry - Anti-RUNX1 / AML1 antibody [EPR23309-113] - BSA and Azide free (ab272458)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling RUNX1 / AML1 with ab272456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272456).

  • Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade – BSA and Azide free (ab272458)
    Anti-RUNX1 / AML1 antibody [EPR23309-113] - ChIP Grade – BSA and Azide free (ab272458)

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