Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15346] to TGF alpha - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TGF alpha antibody [EPR15346] - BSA and Azide free
    See all TGF alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR15346] to TGF alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal skin, fetal liver and breast cancer lysates; HepG2 and Ramos whole cell lysates. IHC-P: Human liver and hepatocellular carcinoma tissues. ICC/IF: HepG2 cells. IP: HepG2 and Ramos whole cell lysates.

  • General notes

    ab224266 is the carrier-free version of ab208156.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling TGF alpha with ab208156 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on the cancer cells of Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling TGF alpha with ab208156 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody  -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab208156 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

  • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells, treated with Brefeldin A (10 µM, 18 hours), labeling TGF alpha with ab208156 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cells, treated with Brefeldin A (10 µM, 18 hours). The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab208156 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

  • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    TGF alpha was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab208156 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208156 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate 10µg (Input).

    Lane 2: ab208156 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab208156 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

  • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    TGF alpha was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab208156 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208156 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Ramos whole cell lysate, 10µg (Input).

    Lane 2: ab208156 IP in Ramos whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab208156 in Ramos whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    This IHC data was generated using the same anti-TGF alpha antibody clone [EPR15346] in a different buffer formulation (cat# ab208156).

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TGF alpha with ab208156 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
    Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

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