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Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22262-22] to ENPP1/PC1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free
    See all ENPP1/PC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22262-22] to ENPP1/PC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 cells.

  • General notes

    ab240832 is the carrier-free version of ab223268 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ENPP1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Immunoprecipitation - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    ENPP1/PC1 was immunoprecipitated from 0.35 mg of MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate using ab223268 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223268 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate 10μg (input)
    Lane 2: ab223268 IP in MDA-MB-231 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223268 in MDA-MB-231 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling ENPP1/PC1 with ab223268 (red) compared with a Rabbit monoclonal IgG  isotype control (ab172730) (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling ENPP1/PC1 (green) with ab223268 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Confocal image showing membranous staining in MDA-MB-231 cells. Tubulin was detected using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (Red). The nuclear countertsain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit (ab150077) at 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    Immunohistochemical analysis of paraffin-embedded human liver tissue stained for ENPP1/PC1 using ab223268 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human liver (PMID: 25539584; PMID: 9344668; PMID: 23861746) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue stained for ENPP1/PC1 using ab223268 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human pancreas (PMID: 9344668; PMID: 23861746) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling ENPP1/PC1 (green) with ab223268 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Confocal image showing membranous staining in HepG2 cells. Tubulin was detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear countertsain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit (ab150077) at 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223268).

  • Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)
    Anti-ENPP1/PC1 antibody [EPR22262-22] - BSA and Azide free (ab240832)

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