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Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15122(B)] to CRTAP/CASP
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CRTAP/CASP antibody [EPR15122(B)]
    See all CRTAP/CASP primary antibodies

  • Description

    Rabbit monoclonal [EPR15122(B)] to CRTAP/CASP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Saos-2, SW1353, A431 and HeLa whole cell lysate (ab150035); Human trachea and lung adenocarcinoma tissue.

  • General notes

     

     

     This product was previously labelled as CRTAP

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    All lanes : Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720) at 1/20000 dilution

    Lane 1 : Saos-2 whole cell lysate
    Lane 2 : SW1353 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720) at 1/5000 dilution + A431 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)

    Immunohistochemical analysis of paraffin embedded Human trachea tissue sections labeling CRTAP/CASP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)

    Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue sections labeling CRTAP/CASP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Immunoprecipitation - Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)

    Lysate from SW1353 cells was immunoprecipitated with ab183720 at a 1/50 dilution. For the subsequent blot, ab183720 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Lane 2: TBS instead of SW1353 lysates. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)
    Anti-CRTAP/CASP antibody [EPR15122(B)] (ab183720)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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