Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Friday March 12, 2021

Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12908(2)] to TAOK1
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TAOK1 antibody [EPR12908(2)]
See all TAOK1 primary antibodies
Description

Rabbit monoclonal [EPR12908(2)] to TAOK1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Rat

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human seminoma and testis whole cell lysate; HeLa, Jurkat, Raw264.7 and NIH3T3 cell line whole cell lysates; Rat brain, heart and spleen tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12908(2)
Isotype

IgG
Research areas

Western blot - Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilution

Lane 1 : Human seminoma lysate
Lane 2 : Human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM /TBST
Western blot - Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/10000 dilution

Lane 1 : HeLa whole (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Exposure time: 1 minute
Western blot - Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lane 4 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Exposure time: 1 minute
Western blot - Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

Immunoprecipitation from Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract using ab197891 at 1/100 dilution for capture (IP +) or Rabbit monoclonal IgG ab172730 (IP -).

For WB ab197891 was used at 1/5000 dilution in 5% NFDM/TBST buffer. Input is Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract. Secondary antibody was anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-TAOK1 antibody [EPR12908(2)] (ab197891)

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