Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 400 µU
Overview
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Product name
Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)
See all ACE2 kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
400 µU -
Assay duration
Multiple steps standard assay -
Product overview
Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273373) utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. We also provide an ACE2 specific inhibitor that can differentiate the ACE2 activity from other proteolytic activity. The kit can detect as low as 0.4 mU. The assay kit is simple and can be used in a high-throughput format.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests ACE2 Assay Buffer 1 x 25ml ACE2 Dilution Buffer 1 x 1.5ml ACE2 Inhibitor (5 mM) 1 x 50µl ACE2 Lysis Buffer 1 x 50ml ACE2 Positive Control 1 x 5µl ACE2 Substrate 1 x 200µl MCA-Standard (1 mM) 1 x 15µl -
Research areas
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
see all
Images
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MCA Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
MCA Standard Curve (0-300 pmol), error bars indicate SD (n=3).
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Kinetic activity curvesKinetic activity curves using different amounts of ACE2 Positive Control in the assay.
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ACE2 Activity in Tissue SamplesACE2 activity was measured for different types of rat tissue samples (total protein in lung and kidney; 17 µg and 23 µg respectively), and human kidney tissue sample (10 µg total protein) in presence (+ Inhibitor) and absence (- Inhibitor) of ACE2 Inhibitor.
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Spiked ACE2 activity and inhibition measured in HEK-293 cell lysateSpiked ACE2 activity and inhibition measured in HEK-293 cell lysate (total protein: 37 µg).