Anti-ETAA1 antibody [EPR14052(2)] (ab197017)
![Anti-ETAA1 antibody [EPR14052(2)] (ab197017)](https://www.abcam.com/ps/products/197/ab197017/Images/ab197017-236628-anti-etaa1-antibody-epr140522-western-blot.jpg "Anti-ETAA1 antibody [EPR14052(2)] (ab197017)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14052(2)] to ETAA1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ETAA1 antibody [EPR14052(2)]
See all ETAA1 primary antibodies -
Description
Rabbit monoclonal [EPR14052(2)] to ETAA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, fetal kidney and fetal liver lysates; MCF7 cell lysate; Mouse brain, mouse heart, rat brain and rat heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14052(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ETAA1 antibody [EPR14052(2)] (ab197017)All lanes : Anti-ETAA1 antibody [EPR14052(2)] (ab197017) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ETAA1 antibody [EPR14052(2)] (ab197017)](https://www.abcam.com/ps/products/197/ab197017/Images/ab197017-236629-anti-etaa1-antibody-epr140522-western-blot.jpg)
Western blot - Anti-ETAA1 antibody [EPR14052(2)] (ab197017)Anti-ETAA1 antibody [EPR14052(2)] (ab197017) at 1/10000 dilution + Human fetal liver lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-ETAA1 antibody [EPR14052(2)] (ab197017)](https://www.abcam.com/ps/products/197/ab197017/Images/ab197017-236631-anti-etaa1-antibody-epr140522-western-blot.jpg)
Western blot - Anti-ETAA1 antibody [EPR14052(2)] (ab197017)All lanes : Anti-ETAA1 antibody [EPR14052(2)] (ab197017) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
-
![Anti-ETAA1 antibody [EPR14052(2)] (ab197017)](https://www.abcam.com/ps/products/197/ab197017/Images/ab197017-4-benefits-of-recombinant-antibodies.png)
Anti-ETAA1 antibody [EPR14052(2)] (ab197017)
