Anti-TEM1 antibody (ab48185)
Key features and details
- Rabbit polyclonal to TEM1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-TEM1 antibody
See all TEM1 primary antibodies -
Description
Rabbit polyclonal to TEM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human TEM1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab48184) -
Positive control
- This antibody gave a positive signal in the following Mouse Tissue Lysates: Skeletal Muscle and Heart
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 80 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 80 kDa).Target
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Function
May play a role in tumor angiogenesis. -
Tissue specificity
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsO-glycosylated with sialylated oligosaccharides.
May be N-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57124 Human
- Entrez Gene: 70445 Mouse
- Omim: 606064 Human
- SwissProt: Q9HCU0 Human
- SwissProt: Q91V98 Mouse
- Unigene: 195727 Human
- Unigene: 29597 Mouse
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Alternative names
- CD164 sialomucin like 1 antibody
- CD164L1 antibody
- CD248 antibody
see all
Images
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Western blot - Anti-TEM1 antibody (ab48185)All lanes : Anti-TEM1 antibody (ab48185) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse)
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 250 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab48185 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TEM1 antibody (ab48185)All lanes : Anti-TEM1 antibody (ab48185) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse)
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 250 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
