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Cancer Tumor immunology Tumor-associated antigens

Anti-TEM1 antibody (ab48185)

Price and availability

244 579 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-TEM1 antibody (ab48185)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TEM1
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-TEM1 antibody
    See all TEM1 primary antibodies
  • Description

    Rabbit polyclonal to TEM1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human TEM1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab48184)

  • Positive control

    • This antibody gave a positive signal in the following Mouse Tissue Lysates: Skeletal Muscle and Heart

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TEM1 protein (ab132505)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab48185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
All applications
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 80 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 80 kDa).

Target

  • Function

    May play a role in tumor angiogenesis.
  • Tissue specificity

    Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications

    O-glycosylated with sialylated oligosaccharides.
    May be N-glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9HCU0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57124 Human
    • Entrez Gene: 70445 Mouse
    • Omim: 606064 Human
    • SwissProt: Q9HCU0 Human
    • SwissProt: Q91V98 Mouse
    • Unigene: 195727 Human
    • Unigene: 29597 Mouse
    • Alternative names

      • CD164 sialomucin like 1 antibody
      • CD164L1 antibody
      • CD248 antibody
      • CD248 antigen endosialin antibody
      • CD248 molecule endosialin antibody
      • CD248_HUMAN antibody
      • Endosialin antibody
      • TEM 1 antibody
      • TEM1 antibody
      • Tumor endothelial marker 1 antibody
      see all

    Images

    • Western blot - Anti-TEM1 antibody (ab48185)
      Western blot - Anti-TEM1 antibody (ab48185)
      All lanes : Anti-TEM1 antibody (ab48185) at 1 µg/ml

      Lane 1 : Skeletal Muscle (Mouse)
      Lane 2 : Heart (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 80 kDa
      Observed band size: 92 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 250 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab48185? Please let us know so that we can cite the reference in this datasheet.

    ab48185 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-TEM1 antibody (ab48185)
      Western blot - Anti-TEM1 antibody (ab48185)
      All lanes : Anti-TEM1 antibody (ab48185) at 1 µg/ml

      Lane 1 : Skeletal Muscle (Mouse)
      Lane 2 : Heart (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 80 kDa
      Observed band size: 92 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 250 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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