Anti-TEM1 antibody (ab67273)
Key features and details
- Rabbit polyclonal to TEM1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEM1 antibody
See all TEM1 primary antibodies -
Description
Rabbit polyclonal to TEM1 -
Host species
Rabbit -
Specificity
ab67273 recognizes both isoforms of TEM1. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.
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Positive control
- WB: Human Colon tissue lysate. HEK-293, A431, A549, and CaCo-2 cell lysates; IHC-P: Human Colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TEM1 antibody (ab67273) at 2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 46, 81 kDa
Observed band size: 46, 90 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehydefixed Human Colon tissue labeling TEM-1 with ab67273 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml
All lanes : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti rabbit secondary antibody
Predicted band size: 46, 81 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
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ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.