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Cancer Tumor immunology Tumor-associated antigens

Anti-TEM1 antibody (ab67273)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-TEM1 antibody (ab67273)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TEM1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TEM1 antibody
    See all TEM1 primary antibodies
  • Description

    Rabbit polyclonal to TEM1
  • Host species

    Rabbit
  • Specificity

    ab67273 recognizes both isoforms of TEM1.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.

  • Positive control

    • WB: Human Colon tissue lysate. HEK-293, A431, A549, and CaCo-2 cell lysates; IHC-P: Human Colon tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Images

  • Western blot - Anti-TEM1 antibody (ab67273)
    Western blot - Anti-TEM1 antibody (ab67273)
    All lanes : Anti-TEM1 antibody (ab67273) at 2 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) cell lysate
    Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution

    Predicted band size: 46, 81 kDa
    Observed band size: 46, 90 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-TEM1 antibody (ab67273)
    Immunocytochemistry/ Immunofluorescence - Anti-TEM1 antibody (ab67273)

    Immunofluorescent analysis of 4% paraformaldehydefixed Human Colon tissue labeling TEM-1 with ab67273 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

  • Western blot - Anti-TEM1 antibody (ab67273)
    Western blot - Anti-TEM1 antibody (ab67273)
    Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
    Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml

    All lanes : Human colon tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : anti rabbit secondary antibody

    Predicted band size: 46, 81 kDa
    Observed band size: 50,80 kDa why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM1 antibody (ab67273)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM1 antibody (ab67273)

    ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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