Anti-MRP4 antibody (ab77184)
Key features and details
- Goat polyclonal to MRP4
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MRP4 antibody
See all MRP4 primary antibodies -
Description
Goat polyclonal to MRP4 -
Host species
Goat -
Specificity
ab77184 is expected to recognize MRP4 isoform 1 (NP_005836.2) only. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding with sequence CNGQPSTLTIFETAL from the C terminal region of the human MRP4 protein (NP_005836.2)
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Positive control
- Lysates prepared from rat renal cortex membranes. Human prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77184 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MRP4 antibody (ab77184)Anti-MRP4 antibody (ab77184) at 0.5 µg/ml + rat renal cortex membranes at 20 µg
Predicted band size: 150 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody (ab77184)ab77184 (4µg/ml) staining of paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody (ab77184)ab77184, at 2.5 µg/ml, staining MRP4 in paraffin embedded human prostate tissue by Immunohistochemistry. Steamed antigen retrievalUse of ab77184 was followed by AP staining.
