Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRP4 antibody [EPR20403] (ab233382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20403] to MRP4
  • Suitable for: IP, IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MRP4 antibody [EPR20403]
    See all MRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20403] to MRP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH:OVCAR-3, A549, LNCaP and U-2 OS whole cell lysates. IHC-P: Human ovarian cancer, kidney and prostate tissues. ICC/IF: LNCaP and A549 cells. IP: U-2 OS whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20403

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MRP4 antibody [EPR20403] (ab233382)
    Western blot - Anti-MRP4 antibody [EPR20403] (ab233382)
    All lanes : Anti-MRP4 antibody [EPR20403] (ab233382) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
    Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg
    Lane 5 : LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 150 kDa
    Observed band size: 220-250 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1-2: 3 minutes, Lane 3: 32 seconds, Lanes 4-5: 2 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with the literature (PMID: 15827327).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human ovarian cancer cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (ab233382)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the LNCaP cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunoprecipitation - Anti-MRP4 antibody [EPR20403] (ab233382)

    MRP4 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with ab233382 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233382 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: U-2 OS whole cell lysate 10 µg (Input).

    Lane 2: ab233382 IP in U-2 OS whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233382 in U-2 OS whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular mass observed is consistent with the literature (PMID: 15827327).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Basolateral membrane staining on human prostate epithelia (PMID:18615486, PMID:25840885). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (ab233382)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. MRP4 is expressed at the apical (brush border) membrane of human kidney tubules (PMID: 11856762). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (ab233382)
    Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (ab233382)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the A549 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Anti-MRP4 antibody [EPR20403] (ab233382)
    Anti-MRP4 antibody [EPR20403] (ab233382)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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