Anti-MRP4 antibody [M4I-10] (ab15602)
![Anti-MRP4 antibody [M4I-10] (ab15602)](https://www.abcam.com/ps/products/15/ab15602/Images/ab15602-312090-anti-mrp4-antibody-m4i-10-western-blot.jpg "Anti-MRP4 antibody [M4I-10] (ab15602)")
Key features and details
- Rat monoclonal [M4I-10] to MRP4
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-MRP4 antibody [M4I-10]
See all MRP4 primary antibodies -
Description
Rat monoclonal [M4I-10] to MRP4 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.
-
Positive control
- Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, 0.7% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
M4I-10 -
Isotype
IgG2a -
Research areas
Images
-
Western blot - Anti-MRP4 antibody [M4I-10] (ab15602)All lanes : Anti-MRP4 antibody [M4I-10] (ab15602) at 20 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCC4 (MRP4) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 159 kDaLanes 1 - 2: Merged signal (red and green). Green - ab15602 observed at 200-250 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab15602 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 (MRP4) knockout cells. Wild-type and ABCC4 (MRP4) knockout samples were subjected to SDS-PAGE. Ab15602 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 20 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)](https://www.abcam.com/ps/products/15/ab15602/Images/ab15602-196912-anti-mrp4-antibody-m4i-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody (ab253031) was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
