Anti-MRP4 antibody (ab32550)
Key features and details
- Rabbit polyclonal to MRP4
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRP4 antibody
See all MRP4 primary antibodies -
Description
Rabbit polyclonal to MRP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MRP4.
Read Abcam's proprietary immunogen policy (Peptide available as ab37589.) -
Positive control
- ab32550 gave a positive signal in the following Human Tissue Lysates: Testis, Spleen, Thymus, Kidney and Prostate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 150 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 150 kDa).Target
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Function
May be an organic anion pump relevant to cellular detoxification. -
Tissue specificity
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10257 Human
- Entrez Gene: 170924 Rat
- Omim: 605250 Human
- SwissProt: O15439 Human
- Unigene: 508423 Human
- Unigene: 13195 Rat
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Alternative names
- ABCC 4 antibody
- ABCC4 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
see all
Images
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Western blot - Anti-MRP4 antibody (ab32550)All lanes : Anti-MRP4 antibody (ab32550) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCC4 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 150 kDa
Observed band size: 150 kDaLanes 1 - 2: Merged signal (red and green). Green - ab32550 observed at 150 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32550 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 knockout cells. Wild-type and ABCC4 knockout samples were subjected to SDS-PAGE. Ab32550 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-MRP4 antibody (ab32550)All lanes : Anti-MRP4 antibody (ab32550) at 1 µg/ml
Lane 1 : Prostate (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 42 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
MRP4 has a predicted molecular weight of 150 kDa; however it has a number of potential transmembrane regions, glycosylation and phosphotylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sells another antibody (ab56675) which detects a band of similar molecular weight in Hela lysate.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab32550 has been referenced in 3 publications.
- Dolezelova E et al. Resveratrol modifies biliary secretion of cholephilic compounds in sham-operated and cholestatic rats. World J Gastroenterol 23:7678-7692 (2017). PubMed: 29209109
- Umapathy A et al. Identification and Functional Characterization of a GSH Conjugate Efflux Pathway in the Rat Lens. Invest Ophthalmol Vis Sci 56:5256-68 (2015). PubMed: 26244301
- Brcakova E et al. Alteration of methotrexate biliary and renal elimination during extrahepatic and intrahepatic cholestasis in rats. Biol Pharm Bull 32:1978-85 (2009). WB ; Rat . PubMed: 19952415
Images
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Western blot - Anti-MRP4 antibody (ab32550)All lanes : Anti-MRP4 antibody (ab32550) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCC4 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 150 kDa
Observed band size: 150 kDaLanes 1 - 2: Merged signal (red and green). Green - ab32550 observed at 150 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32550 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 knockout cells. Wild-type and ABCC4 knockout samples were subjected to SDS-PAGE. Ab32550 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-MRP4 antibody (ab32550)All lanes : Anti-MRP4 antibody (ab32550) at 1 µg/ml
Lane 1 : Prostate (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 42 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
MRP4 has a predicted molecular weight of 150 kDa; however it has a number of potential transmembrane regions, glycosylation and phosphotylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sells another antibody (ab56675) which detects a band of similar molecular weight in Hela lysate.
