Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FXYD1/PLM antibody (ab76597)

Key features and details

  • Rabbit polyclonal to FXYD1/PLM
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FXYD1/PLM antibody
    See all FXYD1/PLM primary antibodies

  • Description

    Rabbit polyclonal to FXYD1/PLM

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human FXYD1/PLM aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88161)

  • General notes

     This product was previously labelled as FXYD1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Cow
Dog
Macaque monkey
Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.

  • Tissue specificity

    Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.

  • Sequence similarities

    Belongs to the FXYD family.

  • Post-translational
    modifications

    Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
    Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FXYD domain containing ion transport regulator 1 antibody
    • FXYD domain-containing ion transport regulator 1 antibody
    • FXYD1 antibody
    • Phospholemman antibody
    • PLM antibody
    • PLM_HUMAN antibody
    see all

Images

  • Western blot - Anti-FXYD1/PLM antibody (ab76597)
    Western blot - Anti-FXYD1/PLM antibody (ab76597)
    All lanes : Anti-FXYD1/PLM antibody (ab76597) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa
    Observed band size: 10 kDa


    Exposure time: 5 minutes

References (6)

Publishing research using ab76597? Please let us know so that we can cite the reference in this datasheet.

ab76597 has been referenced in 6 publications.

  • Ke HY  et al. Changes in cellular Ca2+ and Na+ regulation during the progression towards heart failure in the guinea pig. J Physiol N/A:N/A (2019). PubMed: 30811606
  • Simon MJ  et al. Transcriptional network analysis of human astrocytic endfoot genes reveals region-specific associations with dementia status and tau pathology. Sci Rep 8:12389 (2018). PubMed: 30120299
  • Wanichawan P  et al. Development of a high-affinity peptide that prevents phospholemman (PLM) inhibition of the sodium/calcium exchanger 1 (NCX1). Biochem J 473:2413-23 (2016). WB ; Rat . PubMed: 27247424
  • Hafver TL  et al. Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem 291:4561-79 (2016). PubMed: 26668322
  • Howie J  et al. Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111:17534-9 (2014). PubMed: 25422474
  • Matagne V  et al. Correcting deregulated Fxyd1 expression ameliorates a behavioral impairment in a mouse model of Rett syndrome. Brain Res 1496:104-14 (2013). WB ; Mouse . PubMed: 23246925

Images

  • Western blot - Anti-FXYD1/PLM antibody (ab76597)
    Western blot - Anti-FXYD1/PLM antibody (ab76597)
    All lanes : Anti-FXYD1/PLM antibody (ab76597) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa
    Observed band size: 10 kDa


    Exposure time: 5 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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