Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16082] to FXYD1/PLM
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FXYD1/PLM antibody [EPR16082]
    See all FXYD1/PLM primary antibodies

  • Description

    Rabbit monoclonal [EPR16082] to FXYD1/PLM

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, fetal heart and fetal liver lysates; Mouse and rat heart lysates. IP: Human fetal heart whole cell lysate.

  • General notes

     

     

     This product was previously labelled as FXYD1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

    FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10ug (Input).

    Lane 2: ab200204 IP in Human fetal heart whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
    Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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