Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 10, 2021

Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16082] to FXYD1/PLM
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-FXYD1/PLM antibody [EPR16082]
See all FXYD1/PLM primary antibodies
Description

Rabbit monoclonal [EPR16082] to FXYD1/PLM
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle, fetal heart and fetal liver lysates; Mouse and rat heart lysates. IP: Human fetal heart whole cell lysate.
General notes
This product was previously labelled as FXYD1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16082
Isotype

IgG
Research areas

Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 10 kDa
Observed band size: 12 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: Human fetal heart whole cell lysate 10ug (Input).

Lane 2: ab200204 IP in Human fetal heart whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-FXYD1/PLM antibody [EPR16082] (ab200204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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