Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FXYD1/PLM antibody [EPR16081] (ab191397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16081] to FXYD1/PLM
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FXYD1/PLM antibody [EPR16081]
    See all FXYD1/PLM primary antibodies

  • Description

    Rabbit monoclonal [EPR16081] to FXYD1/PLM

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle lysate; Human fetal heart and fetal liver lysates; Human cardiac muscle tissue.

  • General notes

     

     

     This product was previously labelled as FXYD1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
    Western blot - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
    All lanes : Anti-FXYD1/PLM antibody [EPR16081] (ab191397) at 1/10000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
    Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] (ab191397)

    Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
    Anti-FXYD1/PLM antibody [EPR16081] (ab191397)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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