Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20383] to Myelin Protein Zero
Suitable for: IP, WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-Myelin Protein Zero antibody [EPR20383]
See all Myelin Protein Zero primary antibodies
Description

Rabbit monoclonal [EPR20383] to Myelin Protein Zero
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat sciatic nerve lysates. IHC-P: Human peripheral nerves; Mouse and rat sciatic nerves. IHC-Fr: Mouse and rat sciatic nerves.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20383
Isotype

IgG
Research areas

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

All lanes : Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/5000 dilution

Lane 1 : Mouse sciatic nerve lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat sciatic nerve lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat brain lysate

Lysates/proteins at 4 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 26-28 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

Negative control: liver, brain tissue (PMID 2578885, PMID 25288117).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Immunohistochemical analysis of paraffin-embedded human peripheral nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of human peripheral nerves (PMID: 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Myelin Protein Zero was immunoprecipitated from Human Sciatic Nerve lysate with ab183868 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab183868 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Human Sciatic Nerve lysate 10ug (Input).

Lane 2: ab183868 IP in Human Sciatic Nerve lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183868 in Human Sciatic Nerve lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Immunohistochemical analysis of paraffin-embedded mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

All lanes : Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/1000 dilution

Lane 1 : Human sciatic nerve lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 26-28 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

Negative control: liver tissue (PMID 2578885).
Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Immunohistochemical analysis of paraffin-embedded rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

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