Anti-Myelin Protein Zero antibody (ab39375)
Key features and details
- Chicken polyclonal to Myelin Protein Zero
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgY
Overview
-
Product name
Anti-Myelin Protein Zero antibody
See all Myelin Protein Zero primary antibodies -
Description
Chicken polyclonal to Myelin Protein Zero -
Host species
Chicken -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide; sequence common to mouse and human Myelin Protein Zero.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
IgY fractions were then affinity-purified using a peptide column. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody (ab39375)A tissue section through an adult sciatic nerve. Myelin Protein Zero (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.
-
Western blot - Anti-Myelin Protein Zero antibody (ab39375)All lanes : Anti-Myelin Protein Zero antibody (ab39375) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 25 + 28 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The band observed at 25 kDa could potentially be a cleaved form of Myelin Protein Zero due to the presence of a 29 amino acid signal peptide.
