ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 6 hr
- Sample type: Cell culture extracts, Cell Lysate, Purified mitochondria, Suspension cells, Tissue, Tissue Extracts
Overview
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Product name
ATP synthase Enzyme Activity Microplate Assay Kit
See all ATP Synthase kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Cell Lysate, Purified mitochondria -
Assay type
Enzyme activity -
Assay time
6h 00m -
Species reactivity
Reacts with: Rat, Cow, Human
Does not react with: Mouse -
Product overview
ATP synthase Enzyme Activity Microplate Assay Kit ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.
The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. The conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 96 tests ATP synthase precoated Microtiter plate 1 unit Buffer Tube 1 1 x 10ml Detergent 1 x 1ml Phospholipids 1 x 6ml Reagent Mix 1 x 20ml -
Research areas
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Alternative names
- Complex V
- F1F0
- F1F0 ATP synthase
see all -
Database links
- SwissProt: P25705 Human
- SwissProt: P10719 Rat
Images
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Functional Studies - ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
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Functional Studies - ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)Functional study using ATP synthase Enzyme Activity Microplate Assay Kit (ab109714). -
Functional Studies - ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714). -
Mitochondrial ROS dependent functional deficiency and structural impairment in cardiac mitochondria after sepsis. Image courtesy of Yao X et al.PLoS One. 2015; 10(10): e0139416. doi: 10.1371/journal.pone.0139416.Mitchondiral fractions from the heart tissue of Rats infected by S. pneumoniae, or given PBS sham control, were subjected to measurements of complex I-V activities. Complex I was measured with ab109721 (top left), Complex II + III were measured using ab109905 (top right), Complex IV was measured using ab109911 (bottom left) and Complex V was measured using ab109714 (bottom right).
Freshly isolated mitochondrial pellets were resuspened in PBS supplemented with 10% detergent provided in the kits. Protein concentrations of these mitochondrial lysates were estimated and 25 μg (for complex I, IV and V) or 100 μg (for complex II+III) mitochondrial protein was used per reaction. Enzyme activities were measured spectrophotometricly in triplicate and expressed as changes of absorbance per minute per mg protein.
