Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
Key features and details
- Mouse monoclonal [OTI2D6] to ATP6V0D2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ATP6V0D2 antibody [OTI2D6]
See all ATP6V0D2 primary antibodies -
Description
Mouse monoclonal [OTI2D6] to ATP6V0D2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human ATP6V0D2 aa 1 to the C-terminus. (NP_689778) produced in E.coli.
Database link: Q8N8Y2 -
Positive control
- IHC-P: Human pancreas and kidney tissue. WB: HepG2 cell extracts; pCMV6-ENTRY ATP6V0D2 cDNA transfected HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2D6 -
Isotype
IgG2b -
Research areas
Images
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Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell extracts
Predicted band size: 40 kDa
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Paraffin-embedded human pancreas tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.
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All lanes : Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ATP6V0D2 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa