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Signal Transduction Metabolism Plasma Membrane ATPases

Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI2D6] to ATP6V0D2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-ATP6V0D2 antibody [OTI2D6]
    See all ATP6V0D2 primary antibodies
  • Description

    Mouse monoclonal [OTI2D6] to ATP6V0D2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human ATP6V0D2 aa 1 to the C-terminus. (NP_689778) produced in E.coli.
    Database link: Q8N8Y2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human pancreas and kidney tissue. WB: HepG2 cell extracts; pCMV6-ENTRY ATP6V0D2 cDNA transfected HEK-293T cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI2D6
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases

Images

  • Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell extracts

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)

    Paraffin-embedded human pancreas tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)

    Paraffin-embedded human kidney tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.

  • Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375)
    All lanes : Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY ATP6V0D2 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 40 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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