Anti-Glutathione Reductase antibody (ab87330)
Key features and details
- Rabbit polyclonal to Glutathione Reductase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glutathione Reductase antibody
See all Glutathione Reductase primary antibodies -
Description
Rabbit polyclonal to Glutathione Reductase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Glutathione Reductase aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab95921) -
Positive control
- This antibody gave a positive signal in HeLa and Jurkat whole cell lysates and in the following human tissue lysates: Brain; Liver; Testis; Lung; Heart.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications Common marmosetApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).Target
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Function
Maintains high levels of reduced glutathione in the cytosol. -
Sequence similarities
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
Domain
Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2936 Human
- Omim: 138300 Human
- SwissProt: P00390 Human
- Unigene: 271510 Human
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Alternative names
- GLR1 antibody
- GLUR antibody
- Glutathione reductase antibody
see all
Images
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Western blot - Anti-Glutathione Reductase antibody (ab87330)All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GSR knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDaLanes 1 - 4: Merged signal (red and green). Green - ab87330 observed at 56 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab87330 was shown to recognize Glutathione Reductase in wild-type HAP1 cells as signal was lost at the expected MW in GSR knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSR knockout samples were subjected to SDS-PAGE. Ab87330 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Glutathione Reductase antibody (ab87330)All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : Human testis tissue lysate - total protein (ab30257)
Lane 6 : Lung (Human) Tissue Lysate
Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 37 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab87330 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Glutathione Reductase antibody (ab87330)All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GSR knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDaLanes 1 - 4: Merged signal (red and green). Green - ab87330 observed at 56 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab87330 was shown to recognize Glutathione Reductase in wild-type HAP1 cells as signal was lost at the expected MW in GSR knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSR knockout samples were subjected to SDS-PAGE. Ab87330 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Glutathione Reductase antibody (ab87330)All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : Human testis tissue lysate - total protein (ab30257)
Lane 6 : Lung (Human) Tissue Lysate
Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 37 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
