Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7237] to Glutathione Reductase
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutathione Reductase antibody [EPR7237]
    See all Glutathione Reductase primary antibodies

  • Description

    Rabbit monoclonal [EPR7237] to Glutathione Reductase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in Human Glutathione Reductase (P00390)

  • Positive control

    • Caco-2, A549 or HeLa lysate, Caco-2 and HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. Ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Glutathione Reductase with purified ab128933 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.
  • Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    All lanes : Anti-Glutathione Reductase antibody [EPR7237] (ab128933) at 1/10000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat

    anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Anti-Glutathione Reductase antibody [EPR7237] (ab128933)
    Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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