Anti-VAChT antibody [EPR24248-167] (ab271111)
![Anti-VAChT antibody [EPR24248-167] (ab271111)](https://www.abcam.com/ps/products/271/ab271111/Images/ab271111-1-anti-vacht-antibody-epr24248167-immunohistochemistry-mouse-cerebrum.jpg "Anti-VAChT antibody [EPR24248-167] (ab271111)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24248-167] to VAChT
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-VAChT antibody [EPR24248-167]
See all VAChT primary antibodies -
Description
Rabbit monoclonal [EPR24248-167] to VAChT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatIP MouseRat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cerebrum; Rat cerebrum. IP: Mouse brain tissue lysate; Rat brain tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24248-167 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab271111)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab271111)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly punctate staining in the corpus striatum of mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunoprecipitation - Anti-VAChT antibody (ab271111)VAChT was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab271111 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab271111)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in rat cerebrum. The section was incubated with ab271111 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunoprecipitation - Anti-VAChT antibody (ab271111)VAChT was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: ab271111 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271111 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab271111)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in mouse liver (PMID: 9427309). The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab271111)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat liver. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.