Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Prealbumin antibody [EPR20971] (ab215202)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20971] to Prealbumin
Suitable for: IHC-P, IHC-Fr, WB, IP
Reacts with: Mouse

Product name

Anti-Prealbumin antibody [EPR20971]
See all Prealbumin primary antibodies
Description

Rabbit monoclonal [EPR20971] to Prealbumin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse plasma and serum. IHC-P: Mouse cerebrum and liver tissues. IHC-Fr: Mouse brain (choroid plexus) and liver tissues. IP: Mouse plasma.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20971
Isotype

IgG
Research areas

Western blot - Anti-Prealbumin antibody [EPR20971] (ab215202)

All lanes : Anti-Prealbumin antibody [EPR20971] (ab215202) at 1/1000 dilution

Lane 1 : Mouse plasma
Lane 2 : Mouse serum

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes

Blocking/Dilution: 5% NFDM/TBST
Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (ab215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (choroid plexus) tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the endothelial cells of choroid plexus, negative staining in the endothelial cells of blood vessels on mouse brain tissue section (PMID: 19539719; 20462362).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (ab215202)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in choroid plexuses (arrows) of mouse cerebrum (PMID: 25758461; PMID: 19032346). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (ab215202)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse liver (PMID: 25519307; PMID: 19032346). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (ab215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Prealbumin with ab215202 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the hepatic cells of mouse liver tissue section (PMID: 25519307).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (ab215202)

Prealbumin was immunoprecipitated from 0.35 mg of mouse plasma with ab215202 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215202 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution

Lane 1: Mouse plasma 10 µg (Input).

Lane 2: ab215202 IP in mouse plasma.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215202 in mouse plasma.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.

The band shown at 30 kDa is likely to be a dimer of Prealbumin based on the literature (PMID: 19279259).
Anti-Prealbumin antibody [EPR20971] (ab215202)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com