Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17680] to AKT1 (phospho S124)
Suitable for: ELISA, WB, IP, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-AKT1 (phospho S124) antibody [EPR17680]
See all AKT1 primary antibodies
Description

Rabbit monoclonal [EPR17680] to AKT1 (phospho S124)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, NIH/3T3, C6, RAW264.7 and PC12 whole cell lysates; Human fetal brain and fetal kidney lysates; mouse brain lysate; and rat brain and heart lysates. IP: MCF7 whole cell lysates. ELISA: AKT1 pS124 peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17680
Isotype

IgG
Research areas

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate, untreated
Lane 2 : MCF7 whole cell lysate, treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 15 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate, untreated
Lane 2 : NIH/3T3 whole cell lysate, treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate, untreated
Lane 2 : C6 whole cell lysate, treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Immunoprecipitation of AKT1 from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate achieved using ab183556 at 1/100 dilution.
Lane 1: Input: 10µg of MCF7 whole cell lysate.
Lane 2: MCF7 whole cell lysate following IP with ab183556.
Lane 3: negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab183556 in MCF7 whole cell lysate.
Western blot was performed using ab183556 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.
Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Dot blot analysis of AKT1 (phospho S124) labeled with ab183556 at 1/1000 dilution.
Lane 1: phospho peptide
Lane 2: non phospho peptide
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Exposure time: 3 minutes.
Blocking and Diluting buffer buffer and concentration: 5% NFDM/TBST
ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

ELISA image showing specificity of ab183556 to AKT1 (phospho S124) peptide only.
Peptides concentration: 1000 ng/ml.
ab183556 working dilution: 1/1700.
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500.
Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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