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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT1 antibody [9A4] (ab89402)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-AKT1 antibody [9A4] (ab89402)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [9A4] to AKT1
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-AKT1 antibody [9A4]
    See all AKT1 primary antibodies
  • Description

    Mouse monoclonal [9A4] to AKT1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length active protein

  • Positive control

    • HEK-293 cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.5
    Constituents: 1.21% Tris, 0.75% Glycine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    9A4
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-AKT1 antibody [9A4] (ab89402)
    Western blot - Anti-AKT1 antibody [9A4] (ab89402)

    Primary : All Lanes : Anti AKT1 antibody (ab89402) at 1:2500 dilution. Lane 1 = AKT1 (His tag) full length recombinant protein ab62279 - 50ng. Lane 2 = NIH3T3 serum starved overnight 15ug. Lane 3 = NIH3T3 serum starved overnight and treated with PDGF-AB 50ng/mL for 1 hour 15ug. Secondary : Lanes 1-3 : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Performed under reducing conditions (50mM DTT Sample heated at 60°C). Predicted band size : 56kDa. Observed band size : 56kDa. Blocking step: 5% Milk in 50mM Tris+0.05% Tween for 1 hour at RT. Primary antibody buffer: 5% BSA in 50mM Tris+0.05% Tween overnight. Secondary antibody buffer: 5% Milk in 50mM Tris+0.05% Tween for 2 hours at RT. Exposure time : 5 minutes

  • Western blot - Anti-AKT1 antibody [9A4] (ab89402)
    Western blot - Anti-AKT1 antibody [9A4] (ab89402) This image is courtesy of an anonymous Abreview
    All lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2000 dilution

    All lanes : HEK293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal, undiluted

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-AKT1 antibody [9A4] (ab89402)
    Western blot - Anti-AKT1 antibody [9A4] (ab89402)
    All lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2500 dilution

    Lane 1 : HEK-293 cell lysate at 10 µg
    Lane 2 : HEK-293 cell lysate at 20 µg
    Lane 3 : HEK-293 cell lysate at 40 µg

    Predicted band size: 56 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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