Anti-AKT1 antibody [9A4] (ab89402)
Key features and details
- Mouse monoclonal [9A4] to AKT1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-AKT1 antibody [9A4]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [9A4] to AKT1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length active protein
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Positive control
- HEK-293 cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.5
Constituents: 1.21% Tris, 0.75% Glycine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9A4 -
Isotype
IgG1 -
Research areas
Images
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Primary : All Lanes : Anti AKT1 antibody (ab89402) at 1:2500 dilution. Lane 1 = AKT1 (His tag) full length recombinant protein ab62279 - 50ng. Lane 2 = NIH3T3 serum starved overnight 15ug. Lane 3 = NIH3T3 serum starved overnight and treated with PDGF-AB 50ng/mL for 1 hour 15ug. Secondary : Lanes 1-3 : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Performed under reducing conditions (50mM DTT Sample heated at 60°C). Predicted band size : 56kDa. Observed band size : 56kDa. Blocking step: 5% Milk in 50mM Tris+0.05% Tween for 1 hour at RT. Primary antibody buffer: 5% BSA in 50mM Tris+0.05% Tween overnight. Secondary antibody buffer: 5% Milk in 50mM Tris+0.05% Tween for 2 hours at RT. Exposure time : 5 minutes
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All lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2000 dilution
All lanes : HEK293 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal, undiluted
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2500 dilution
Lane 1 : HEK-293 cell lysate at 10 µg
Lane 2 : HEK-293 cell lysate at 20 µg
Lane 3 : HEK-293 cell lysate at 40 µg
Predicted band size: 56 kDa