Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2109Y] to AKT1 (phospho S473) - BSA and Azide free
  • Suitable for: Dot blot, In-Cell ELISA, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free
    See all AKT1 primary antibodies

  • Description

    Rabbit monoclonal [EP2109Y] to AKT1 (phospho S473) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    AKT1 (phospho S473) antibody (ab81283) detects AKT1 phosphorylated at Serine 473. The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue.

  • Tested applications

    Suitable for: Dot blot, In-Cell ELISA, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa (grown in serum free media overnight, then treated with 150nM Insulin for 5min) whole cell lysate; MCF7 (treated with CCCP) whole cell lysate; LNCaP (treated with 100nM Cacyculin A for 30min) whole cell lysate; NIH/3T3 (treated with PDGF) whole cell lysate; PC-12 whole cell lysate. IHC-P: Human cervical carcinoma tissue. IHC-Fr: Human peritoneal tumor tissue.

  • General notes

    ab215873 is the carrier-free version of ab81283. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes. 

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab215873 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)

    ab81283, at 1/100 dilution, staining AKT1 in untreated (left panel) and Phosphatase-treated (right panel) human cervical carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81283).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873) Image from Henken FE et al. Mol Cancer. 2011 Jun 10;10:71. Fig 7.; doi:10.1186/1476-4598-10-71; 10 June 2011 Molecular Cancer 2011 10:71.

    Immunohistochemical analysis of Human HPV16 immortalized keratinocytes transfected with non-targeting siRNA, staining AKT1 (phospho S473) (green) with ab81283.
    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Samples were blocked with 10% goat serum before incubating with primary antibody (1/100). Fluoroscein-conjugated tyramide was used to detect staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81283).

  • Dot Blot - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    Dot Blot - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)

    Dot blot analysis of AKT1 (phospho S473) phospho peptide (Lane 1) and AKT1 non-phospho peptide (Lane 2) labelling AKT1 (phospho S473) phospho peptide with ab81283 at a dilution of 1:1000 dilution (0.259μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81283).

  • In-Cell ELISA - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    In-Cell ELISA - Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    NIH3T3 cells were starved overnight and treated with PDGF 50ng/mL or vehicle control for 1 hour prior to fixation with 4% paraformaldehyde. Levels of total Akt were measured using antibody ab81283 on an infrared in cell ELISA assay platform.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81283).

  • Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
    Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)

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