Anti-AKT1 antibody [M1843] (ab108202)
![Anti-AKT1 antibody [M1843] (ab108202)](https://www.abcam.com/ps/products/108/ab108202/Images/ab108202-450623-anti-akt1-antibody-m1843-western-blot-hela-mcf7-daudi-lysates.jpg "Anti-AKT1 antibody [M1843] (ab108202)")
Key features and details
- Mouse monoclonal [M1843] to AKT1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-AKT1 antibody [M1843]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [M1843] to AKT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7, Daudi, HeLa, A431 or A549 cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
This mouse monoclonal to AKT1 has been knockout validated in Western blot. The expected band for AKT1 was observed in wild type cells and the band was not seen in knockout cells.
This product was manufactured under U.S. Patent No. 5,675,063.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
M1843 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-AKT1 antibody [M1843] (ab108202)All lanes : Anti-AKT1 antibody [M1843] (ab108202) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : AKT1 knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1- 4: Merged signal (red and green). Green - ab108202 observed at 60 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab108202 was shown to react with AKT1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264852 (knockout cell lysate ab256835) was used. Wild-type HeLa and AKT1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108202 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AKT1 antibody [M1843] (ab108202)](https://www.abcam.com/ps/products/108/ab108202/Images/ab108202-251554-anti-akt1-antibody-m1843-western-blot.jpg)
Western blot - Anti-AKT1 antibody [M1843] (ab108202)Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: AKT1 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target - ab108202 observed at 55 kDa
Lanes 3 and 4: Red signal from loading control - ab8227 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab108202 detected the expected band for AKT1 in wild-type cells and the band was not seen in knockout cells. Wild-type and AKT1 knockout samples were subjected to SDS-PAGE. ab108202 and ab8227 (loading control to beta actin) were both diluted at 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
