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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)

Price and availability

328 339 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6150] to AKT1 (phospho S129)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-AKT1 (phospho S129) antibody [EPR6150]
    See all AKT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6150] to AKT1 (phospho S129)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human AKT1 aa 100-200 (phospho S129).
    Database link: P31749
    (Peptide available as ab204134)

  • Positive control

    • MCF-7 cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6150
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
    Western blot - Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
    All lanes : Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : MCF-7 cell lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 55 kDa

  • OI-RD Scanning - Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
    OI-RD Scanning - Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)
    Anti-AKT1 (phospho S129) antibody [EPR6150] (ab133458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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