Anti-AKT1 (phospho S473) antibody (ab8932)
Key features and details
- Rabbit polyclonal to AKT1 (phospho S473)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 (phospho S473) antibody
See all AKT1 primary antibodies -
Description
Rabbit polyclonal to AKT1 (phospho S473) -
Host species
Rabbit -
Specificity
The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: CHFPQFSpYSAS conjugated to KLH, corresponding to C terminal amino acids 468-477 of Human AKT.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 (phospho S473) antibody (ab8932)
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Normal human breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
This image is a magnification of the accompanying breast tumor image.
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932) Image from Meyer S et al., PLoS One. 2012;7(6):e38222. Epub 2012 Jun 7. Fig S5.; doi:10.1371/journal.pone.0038222; June 7, 2012, PLoS ONE 7(6): e38222.Immunohistochemical analysis of Human prostate cancer and melanoma cell lines, staining AKT1 (phospho S473) with ab8932. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
