Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)
![Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)](https://www.abcam.com/ps/products/108/ab108266/Images/ab108266-120780-anti-akt1-phospho-t450-antibody-epr4157-immunohistochemistry.gif "Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4157] to AKT1 (phospho T450)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-AKT1 (phospho T450) antibody [EPR4157]
See all AKT1 primary antibodies -
Description
Rabbit monoclonal [EPR4157] to AKT1 (phospho T450) -
Host species
Rabbit -
Specificity
ab108266 only detects AKT1 phosphorylated at threonine 450. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
A phospho specific peptide corresponding to residues surrounding threonine 450 of Human AKT1.
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Positive control
- NIH3T3 cell lysate; Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4157 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)
ab108266 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)](https://www.abcam.com/ps/products/108/ab108266/Images/ab108266-120781-anti-akt1-phospho-t450-antibody-epr4157-western-blot.jpg)
Western blot - Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate, untreated
Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)](https://www.abcam.com/ps/products/108/ab108266/Images/ab108266-3-benefits-of-recombinant-antibodies.png)
Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266)
