Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4423] to CD33 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD33 antibody [EPR4423] - BSA and Azide free
    See all CD33 primary antibodies

  • Description

    Rabbit monoclonal [EPR4423] to CD33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP1 cell lysate, U937 cell lysate, HL 60 cell lysate, HepG2 cell lysate, HeLa cell lysate Flow Cyt: THP1 cells

  • General notes

    ab214439 is the carrier-free version of ab134115. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    214439 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)
    Flow Cytometry - Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP1 (Human monocytic leukemia monocyte, Right) labeling CD33 with ab134115 at 1/500 dilution (red). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).
     
    Negative control: Jurkat.
    Gated on viable cells.
     
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134115).
  • Immunoprecipitation - Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)
    Immunoprecipitation - Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)

    ab134115 (purified) at 1/60 immunoprecipitating CD33 in 10 μg THP-1 (Lanes 1 and 2, observed at 67-75 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134115).

  • Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)
    Anti-CD33 antibody [EPR4423] - BSA and Azide free (ab214439)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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