Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR061G] to CD33
Suitable for: IP, WB, IHC-P
Reacts with: Human

Product name

Anti-CD33 antibody [BLR061G]
See all CD33 primary antibodies
Description

Rabbit monoclonal [BLR061G] to CD33
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CD33 aa 314-364 (C terminal). The exact sequence is proprietary.
Database link: P20138
Positive control
- IHC-P: Acute Myeloid leukemia tissue. WB: HEL 921.7, My-La CD4+ and THP-1 whole cell lysates. IP: HEL921.7 whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR061G
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD33 antibody [BLR061G] (ab264414)

CD33 was immunoprecipitated from HEL 92.1.7 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264414 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab264414 at 1/1000 dilution.

Lane 1: ab264414 IP in HEL 92.1.7 cell lysate.
Lane 2: Control IgG IP in HEL 92.1.7 cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.

Lysates prepared using NETN lysis buffer.
Western blot - Anti-CD33 antibody [BLR061G] (ab264414)

All lanes : Anti-CD33 antibody [BLR061G] (ab264414) at 1/1000 dilution

Lane 1 : HEL 92.1.7 whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : My-La CD4+ whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : THP-1 (human monocytic leukemia cell line) whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 40 kDa

Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [BLR061G] (ab264414)

Formalin-fixed, paraffin-embedded Acute Myeloid Leukemia tissue stained for CD33 using ab264414 at 1/200 dilution in immunohistochemical analysis. The secondary was an HRP-conjugated goat anti-rabbit IgG antibody. Substrate: DAB.
Anti-CD33 antibody [BLR061G] (ab264414)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com