Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

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Anti-CD33 antibody [EPR23052-5] (ab264558)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23052-5] to CD33
Suitable for: Flow Cyt, IP
Reacts with: Human

Product name

Anti-CD33 antibody [EPR23052-5]
See all CD33 primary antibodies
Description

Rabbit monoclonal [EPR23052-5] to CD33
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

IP
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow Cyt: THP-1 and Human peripheral blood mononuclear cell cells. IP: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
IP	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23052-5
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD33 antibody [EPR23052-5] (ab264558)

CD33 was immunoprecipitated from 0.35 mg THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug with ab264558 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264558 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug

Lane 2: ab264558 IP in THP-1 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264558 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Flow Cytometry - Anti-CD33 antibody [EPR23052-5] (ab264558)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD33 with ab264558 at 1/50 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab264558 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor^® 647 (PMID: 26468886).

Gated on viable cells.
Flow Cytometry - Anti-CD33 antibody [EPR23052-5] (ab264558)

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling CD33 with ab264558 at 1/50 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control: Jurkat (PMID:30519686).
Anti-CD33 antibody [EPR23052-5] (ab264558)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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