Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Wednesday March 10, 2021

Anti-p53 (phospho S6) antibody [Y179] (ab32132)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y179] to p53 (phospho S6)
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-p53 (phospho S6) antibody [Y179]
See all p53 primary antibodies
Description

Rabbit monoclonal [Y179] to p53 (phospho S6)
Host species

Rabbit
Specificity

ab32132 detects p53 phosphorylation on Serine 6. Predicted to react with p53 beta and gamma isoform, based on sequence homology.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human p53 aa 1-100 (phospho S6). The exact sequence is proprietary.
Database link: P04637
Positive control
- WB: MCF7 cell lysate. IHC-P: human urinary bladder carcinoma. IP: HeLa cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

Y179
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using ab32132 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

All lanes : Anti-p53 (phospho S6) antibody [Y179] (ab32132) at 1/1000 dilution

Lane 1 : HepG2 whole cell lysate - untreated
Lane 2 : HepG2 whole cell lysate - treated with Etopside

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 53 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

Purified ab32132 at 1/50 dilution (2µg) immunoprecipitating p53 in HeLa treated with etoposide whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with etoposide whole cell lysate 10µg
Lane 2 (+): ab32132 + HeLa treated with etoposide whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32132 in HeLa treated with etoposide whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

ab32132 showing positive staining in Lung adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-p53 (phospho S6) antibody [Y179] (ab32132) This image is courtesy of an Abreview submitted by Christian Marx

All lanes : Anti-p53 (phospho S6) antibody [Y179] (ab32132) at 1/500 dilution

Lane 1 : DMSO treated HCT116 cell line
Lane 2 : 10 µM irinotecan (CPT-11) treated HCT116 cell line for 24 hours

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772) at 1/1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Exposure time: 3 minutes

Blocking: 1 hour at room temperature with 5% Milk in TBST.
Diluent: 2% Milk in TBST
13% SDS-PAGE gel used.

Secondary antibody - goat anti-rabbit Alexa Fluor 680
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

ab32132 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

All lanes : Anti-p53 (phospho S6) antibody [Y179] (ab32132) at 1/5000 dilution

Lane 1 : MCF7 cell lysate, non-treated
Lane 2 : MCF7 cell lysate, treated with Actinomycin D for 3 hours
Lane 3 : MCF7 cell lysate, treated with Actinomycin D for 6 hours
Lane 4 : MCF7 cell lysate, treated with Actinomycin D for 18 hours

Predicted band size: 44 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] (ab32132)

ab32132 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-p53 (phospho S6) antibody [Y179] (ab32132)

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