Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR358(2)] to p53 (acetyl K382)
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (acetyl K382) antibody [EPR358(2)]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR358(2)] to p53 (acetyl K382)

  • Host species

    Rabbit

  • Specificity

    ab75754 only detects p53 acetylated on Lysine 382.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human p53 aa 350 to the C-terminus (acetyl K382). The exact sequence is proprietary.
    Database link: P04637
    (Peptide available as ab183615)

  • Positive control

    • WB: HepG2 cell lysates treated with etopside and TSA. ICC/IF: HeLa cells Flow Cyt: HepG2 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899

  • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/1000 dilution (Purified)

    Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
    Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899

  • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/5000 dilution (unpurified)

    Lane 1 : HepG2 cell lysates un-treated
    Lane 2 : HepG2 cell lysates treated with etopside and TSA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for 4 hours cells labeling p53 with purified ab75754 at 1/250 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Flow Cytometry - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) starved overnight, then treated with 30ug/ml etoposide for 8 hours followed by 500ng/ml TSA for 4 hours cells labeling p53 with purified ab75754 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
    Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

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