Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [POE316A] to p53
Suitable for: WB
Reacts with: Mouse

Product name

Anti-p53 antibody [POE316A]
See all p53 primary antibodies
Description

Rat monoclonal [POE316A] to p53
Host species

Rat
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment within Mouse p53. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P02340

Run BLAST with Run BLAST with
Positive control
- WB: LLC1 AND RAW264.7 whole cell lysate; IHC-P: Mouse fibrosarcoma and thymus.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Ion Exchange Chromatography
Clonality

Monoclonal
Clone number

POE316A
Isotype

IgG2a
Research areas

Western blot - Anti-p53 antibody [POE316A] (ab241566)

All lanes : Anti-p53 antibody [POE316A] (ab241566) at 1/1000 dilution

Lane 1 : LLC1 (mouse lung carcinoma), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 103 secs.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [POE316A] (ab241566) Giovanna Roncador, Spanish Nacional Cancer Research Centre (CNIO)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Wild type mouse fibrosarcoma labeling p53 with ab241566 at 1/50 dilution.

Image A: nuclear staining on Wild type mouse fibrosarcoma

Image B: nearly no staining on p53 knock-out mouse fibrosarcoma

The experiment was done in Discovery XT, Ventana-Roche machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [POE316A] (ab241566) Giovanna Roncador, Spanish Nacional Cancer Research Centre (CNIO)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thymus labeling p53 with ab241566 at 1/50 dilution.

The experiment was done in Discovery XT, Ventana-Roche machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
Anti-p53 antibody [POE316A] (ab241566)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com