HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)
![HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)](https://www.abcam.com/ps/products/196/ab196980/Images/ab196980-242020-anti-fibrillarin-antibody-epr10823b-nucleolar-marker-hrp-western-blot.jpg "HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
See all Fibrillarin primary antibodies -
Description
HRP Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK293, HeLa, HepG2 and MOLT4 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10823(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)All lanes : HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980) at 1/5000 dilution
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196980 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)](https://www.abcam.com/ps/products/196/ab196980/Images/ab196980-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)
