Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free
See all p53R2+RRM2 primary antibodies
Description

Rabbit monoclonal [EPR20651] to p53R2+RRM2 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251513 is the carrier-free version of ab209995. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251513 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR20651
Isotype

IgG
Research areas

Western blot - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

All lanes : Anti-p53R2 + RRM2 antibody [EPR20651] (ab209995) at 1/10000 dilution

Lane 1 : His-tagged human RRM2 recombinant protein, 10 ng
Lane 2 : His-tagged human p53R2 recombinant protein, 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45 kDa
Observed band size: 38,39 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

This data was developed using ab209995, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

This data was developed using ab209995, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RRM2/RRM2B with ab209995 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and weak nucleus staining on epithelial cells of human colon (PMID: 23002339). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

All lanes : Anti-p53R2 + RRM2 antibody [EPR20651] (ab209995) at 1/1000 dilution

Lane 1 : Human ovary cancer at 20 µg
Lane 2 : Human thyroid cancer at 20 µg
Lane 3 : Rat brain at 20 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 5 : U-2 OS (Human bone osteosarcoma epithelial cell) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 45 kDa
Observed band size: 41,45 kDa why is the actual band size different from the predicted?

This data was developed using ab209995, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1-3: 3 minutes; Lanes 4-5: 15 seconds.

The MW of RRM2 and p53R2 observed is consistent with the literature (PMID 14661056; PMID 15670581; PMID 25213022).
Western blot - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

All lanes : Anti-p53R2 + RRM2 antibody [EPR20651] (ab209995) at 1/1000 dilution

Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45 kDa
Observed band size: 41,45 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

This data was developed using ab209995, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The MW of RRM2 and p53R2 observed is consistent with the literature (PMID: 14661056; 15670581; 25213022).
Western blot - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

Anti-p53R2 + RRM2 antibody [EPR20651] (ab209995) at 1/1000 dilution + 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 45 kDa
Observed band size: 41,45 kDa why is the actual band size different from the predicted?

This data was developed using ab209995, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The MW of RRM2 and p53R2 observed is consistent with the literature (PMID: 14661056; 15670581; 25213022).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

This data was developed using ab209995, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling RRM2/RRM2B with ab209995 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID: 25838540). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

This data was developed using ab209995, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RRM2+ p53R2 with ab209995 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells (PMID: 194169810). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

This data was developed using ab209995, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling RRM2+ p53R2 with ab209995 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)

This data was developed using ab209995, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 80% methanol fixed, 0.1% Tween-20 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling p53R2 + RRM2 with ab209995 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free (ab251513)