Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP102] to Cytokeratin 8 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free
    See all Cytokeratin 8 primary antibodies

  • Description

    Rabbit monoclonal [SP102] to Cytokeratin 8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast ductal carcinoma, breast, lung squamous cell carcinoma, placenta, thyroid, bladder, bladder transitional cell carcinoma, stomach, stomach adenocarcinoma, prostate, prostate adenocarcinoma and lung tissues. Flow Cyt: HaCaT cells. IHC-Fr: Rat and mouse small intestine tissue ICC/IF: HaCaT cells.

  • General notes

    ab240986 is the carrier-free version of ab227644.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse small intestine tissue sections labeling Cytokeratin 8 with Purified ab227644 at 1:20 (6.85 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1:25 (5.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat small intestine tissue sections labeling Cytokeratin 8 with Purified ab227644 at 1:20 (6.85 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
  • Flow Cytometry - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Flow Cytometry - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Flow Cytometry analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1:200 dilution (0.685 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

    Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227644)

  • Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
    Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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