Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
Key features and details
- Mouse monoclonal [K8.8] to Cytokeratin 8
- Suitable for: IHC-P, Flow Cyt, WB, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 8 antibody [K8.8]
See all Cytokeratin 8 primary antibodies -
Description
Mouse monoclonal [K8.8] to Cytokeratin 8 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Full length native protein (purified) corresponding to Human Cytokeratin 8. Keratin preparation from a human carcinoma
Database link: P05787 -
Positive control
- WB: HCT116 cell lysate. IHC: Human Colon Carcinoma tissue. ICC: HCT116 cells. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
K8.8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labeling Cytokeratin 8 with ab193346 at 0.5 μg/ml
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Immunocytochemical analysis of HCT116 cells labeling Cytokeratin 8 with ab193346 at 2 μg/ml followed by Goat anti mouse IgG CF488 (green). The nuclear counterstain used reddot (red).
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Anti-Cytokeratin 8 antibody [K8.8] (ab193346) at 2 µg/ml + HCT116 cell lysate
Predicted band size: 53 kDa
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 8 with ab193346 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).