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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 8 antibody [K8.8] (ab193346)

Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [K8.8] to Cytokeratin 8
  • Suitable for: IHC-P, Flow Cyt, WB, ICC
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Cytokeratin 8 antibody [K8.8]
    See all Cytokeratin 8 primary antibodies
  • Description

    Mouse monoclonal [K8.8] to Cytokeratin 8
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Human Cytokeratin 8. Keratin preparation from a human carcinoma
    Database link: P05787

  • Positive control

    • WB: HCT116 cell lysate. IHC: Human Colon Carcinoma tissue. ICC: HCT116 cells. Flow Cyt: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    K8.8
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)

    Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labeling Cytokeratin 8 with ab193346 at 0.5 μg/ml

  • Immunocytochemistry - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
    Immunocytochemistry - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)

    Immunocytochemical analysis of HCT116 cells labeling Cytokeratin 8 with ab193346 at 2 μg/ml followed by Goat anti mouse IgG CF488 (green). The nuclear counterstain used reddot (red).

  • Western blot - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
    Western blot - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
    Anti-Cytokeratin 8 antibody [K8.8] (ab193346) at 2 µg/ml + HCT116 cell lysate

    Predicted band size: 53 kDa

  • Flow Cytometry - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)
    Flow Cytometry - Anti-Cytokeratin 8 antibody [K8.8] (ab193346)

    Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 8 with ab193346 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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