Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-heavy chain Myosin/MYH3 antibody (ab124205)

Key features and details

  • Rabbit polyclonal to heavy chain Myosin/MYH3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-heavy chain Myosin/MYH3 antibody
    See all heavy chain Myosin/MYH3 primary antibodies

  • Description

    Rabbit polyclonal to heavy chain Myosin/MYH3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Dog, Pig

  • Immunogen

    Synthetic peptide corresponding to Human heavy chain Myosin/MYH3 aa 100-200 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates. IHC: Mouse Skeletal Muscle.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P
Use a concentration of 1 µg/ml.

Target

  • Function

    Muscle contraction.

  • Involvement in disease

    Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
    Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.

  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • Developmental stage

    Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle.

  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • embryonic antibody
    • fast skeletal muscle antibody
    • HEMHC antibody
    • Muscle embryonic myosin heavy chain 3 antibody
    • Muscle embryonic myosin heavy chain antibody
    • MYH 3 antibody
    • Myh3 antibody
    • MYH3_HUMAN antibody
    • MYHC EMB antibody
    • MYHSE 1 antibody
    • MYHSE1 antibody
    • Myosin heavy chain 3 antibody
    • Myosin heavy chain 3 skeletal muscle embryonic antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain fast skeletal muscle embryonic antibody
    • Myosin Heavy Polypeptide 3 antibody
    • Myosin heavy polypeptide 3 skeletal muscle embryonic antibody
    • Myosin skeletal heavy chain embryonic 1 antibody
    • Myosin-3 antibody
    • SMHCE antibody
    see all

Images

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody (ab124205)

    IHC image of Anti-heavy chain Myosin/MYH3 antibody staining in a section of formalin-fixed paraffin-embedded mouse skeletal muscle performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab124205, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Heart (Human) Tissue Lysate - fetal normal tissue
    Lane 6 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 190 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References (13)

Publishing research using ab124205? Please let us know so that we can cite the reference in this datasheet.

ab124205 has been referenced in 13 publications.

  • Ling X  et al. Lidocaine Inhibits Myoblast Cell Migration and Myogenic Differentiation Through Activation of the Notch Pathway. Drug Des Devel Ther 15:927-936 (2021). PubMed: 33688167
  • Yu JA  et al. LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B. Cell Death Discov 7:73 (2021). PubMed: 33837177
  • Huang C  et al. Evidence Against the Causal Relationship Between a Putative Cis-Regulatory Variant of MYH3 and Intramuscular Fat Content in Pigs. Front Vet Sci 8:672852 (2021). PubMed: 34150892
  • Shahid M  et al. Pioglitazone Alters the Proteomes of Normal Bladder Epithelial Cells but Shows No Tumorigenic Effects. Int Neurourol J 24:29-40 (2020). PubMed: 32252184
  • Xia Q  et al. Flavonoids Sophoranone Promotes Differentiation of C2C12 and Extraocular Muscle Satellite Cells. Ophthalmic Res N/A:N/A (2020). PubMed: 32344402
View all Publications for this product

Images

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody (ab124205)

    IHC image of Anti-heavy chain Myosin/MYH3 antibody staining in a section of formalin-fixed paraffin-embedded mouse skeletal muscle performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab124205, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody (ab124205)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Heart (Human) Tissue Lysate - fetal normal tissue
    Lane 6 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 190 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

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