Anti-MEK1 (phospho T286) antibody (ab129431)
Key features and details
- Rabbit polyclonal to MEK1 (phospho T286)
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEK1 (phospho T286) antibody
See all MEK1 primary antibodies -
Description
Rabbit polyclonal to MEK1 (phospho T286) -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Elephant
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Immunogen
Synthetic peptide corresponding to amino acids surrounding Threonine 286 of Human MEK1 when the Threonine residue is phosphorylated (NP_002746.1).
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Positive control
- HeLa whole cell lysate, treated with nocodazole.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab129431 was affinity purified using an epitope specific to Phospho MEK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129431)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/1000 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129431)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/5000 (0.2µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MEK1 (phospho T286) antibody (ab129431)Detection of MEK1 by Western Blot of Immunprecipitate.
ab129431 at 1µg/ml (lanes 1-4) or Control IgG (lanes 5-6)staining MEK1 in HeLa whole cell lysate immunoprecipitated using ab129431 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 1: HeLa whole cell lysate, mock treated.
Lane 2: HeLa whole cell lysate, nocodazole treated.
Lane 3: Immunoprecipitation of MEK1 (phospho T286) from mock treated HeLa whole cell lysate.
Lane 4: Immunoprecipitation of MEK1 (phospho T286) from nocodazole treated HeLa whole cell lysate.
Lane 5: HeLa whole cell lysate, mock treated.
Lane 6: HeLa whole cell lysate, nocodazole treated.
Predicted band size : 43 kDa.
Detection: Chemiluminescence with exposure time 10 seconds.
