Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)
![Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)](https://www.abcam.com/ps/products/77/ab77869/Images/Cytokeratin-3-Primary-antibodies-ab77869-2.jpg "Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)")
Key features and details
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 3/CK-3 antibody [AE5]
See all Cytokeratin 3/CK-3 primary antibodies -
Description
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3.
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General notes
Previously labelled as Cytokeratin 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AE5 -
Myeloma
P3-x63-Ag8 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)
Overlay histogram showing A431 cells stained with ab77869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77869, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
