Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)
![Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)](https://www.abcam.com/ps/products/8/ab8741/Images/ab8741-368198-AntiCytokeratin-16-K16-antibody-LL025-immunohistochemistry-esophagus-human.jpg "Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [LL025] to Cytokeratin 16/K16
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 16/K16 antibody [LL025]
See all Cytokeratin 16/K16 primary antibodies -
Description
Mouse monoclonal [LL025] to Cytokeratin 16/K16 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide within Human Cytokeratin 16/K16. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P08779 -
Positive control
- IHC-P: Human esophagus and tonsil tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomas -
Clonality
Monoclonal -
Clone number
LL025 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)
Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16/K16 with ab8741 at 2.74µg/ml, followed by ready to use secondary. Positive staining on human esophagus tissue is observed. The section was incubated with ab8741 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)](https://www.abcam.com/ps/products/8/ab8741/Images/ab8741-368197-AntiCytokeratin-16-K16-antibody-LL025-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 16/K16 with ab8741 at 2.74µg/ml, followed by ready to use secondary. Positive staining on epithelial cells of human tonsil tissue is observed. The section was incubated with ab8741 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)](https://www.abcam.com/ps/products/8/ab8741/Images/ab8741-6-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 16/K16 antibody [LL025] (ab8741)
