Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6508(N)] to LXR alpha
Suitable for: Flow Cyt, WB, ICC/IF
Reacts with: Human

Product name

Anti-LXR alpha antibody [EPR6508(N)]
See all LXR alpha primary antibodies
Description

Rabbit monoclonal [EPR6508(N)] to LXR alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LXR alpha aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q13133
(Peptide available as ab191835)
Positive control
- Human fetal liver, Jurkat and MCF-7 cell lysates. Jurkat cells and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6508(N)
Isotype

IgG
Research areas

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323) Image is courtesy of an anonymous AbReview.

All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

All lanes : Human SCC61 whole cell lysates

Lysates/proteins at 23 µg per lane.

Secondary
All lanes : Rabbit anti-rabbit polyclonal HRP conjugate at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 50 kDa
Additional bands at: 100 kDa (possible non-specific binding)

Exposure time: 1 second

From left to right lanes 1 - 3. Blocking was done with 5% milk in PBS incubated for 1 hour at 22°C.
See Abreview
Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Immunofluorescent staining of Jurkat cells labeling LRX alpha using ab176323 at a 1/50 dilution.
Flow Cytometry - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Flow cytometric analysis of permeabilized MCF-7 cells labeling LXR alpha using ab176323 at a 1/10 dilution (red) or a rabbit IgG control (green).
Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

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